Adenylyl cyclase isoforms in the vestibular periphery of the rat

The expression of adenylyl cyclase (AC) isoforms in the adult rat vestibula r periphery was investigated using reverse transcription polymerase chain r eaction (RT-PCR). AC II, TV and V mRNAs were expressed in both Scarpa's gan glion and vestibular end organs. In addition, in the vestibular end organs, an AC mRNA not previously reported in the rat was identified. The cloned s equence (GenBank accession no. AF184150) represented 95 amino acids with 10 0% similarity to the human AC VII and 94% to the bovine AC VII. AC VII mRNA also was found in the cerebellum but was undetectable in heart, kidney, li ver and Scarpe's ganglion. (C) 2000 Published by Elsevier Science B.V. All rights reserved.