Complex cytogenetic abnormalities in T-lymphoblastic lymphoma: Resolution by spectral karyotyping

We describe a case of T-lymphoblastic lymphoma (T-LBL) in a 13-year-old boy in in which conventional cytogenetic analysis of lymph node tissue show ed complex karyotypic aberrations including add(1p), add(2q), and multiple ch romosomal deletions involving 5q, 7p, 7q, 13q, and 16q. Analysis by Spectra l karyotyping (SKY) refined add(1p) to a paracentric inversion of Ip, and a dd(2q) to an unbalanced translocation between chromosomes 2 and 4, The chro mosomal deletions rr ere simple deletions except del(5q), which ri as confi rmed by SKY to Dc a cryptic unbalanced translocation between chromosomes 5 and 18. The present report illustrates that SKY technology is useful in ide ntifying subtle translocations and resolving complex karyotypic aberrations in neoplastic disorders. (C) Elsevier Science Inc., 2000. All rights reser ved.