Impaired bone formation in transgenic mice resulting from altered integrinfunction in osteoblasts

To determine the role of integrins in mature osteoblasts in vivo, we expres sed in transgenic mice a dominant-negative integrin subunit (beta 1-DN) con sisting of the beta 1 subunit cytoplasmic and transmembrane domains, driven by the osteoblast-specific osteocalcin promoter. Immature osteoblasts isol ated ham transgenic animals differentiated normally in vitro until the oste ocalcin promoter became active; thereafter they detached from the substratu m, suggesting that beta 1-DN was impairing adhesion in mature osteoblasts. Transgenic animals had reduced bone mass, with increased cortical porosity in long bones and thinner hat bones in the skull. At 35 days, the rate of b one formation was reduced in cortical bone, and the parietal banes were 45% thinner than in wild-type animals. Active osteoblasts were less polar and had larger areas of cytoplasm with intracelluar stores of matrix molecules. Osteocyte lacunae appeared normal around the cell body but did not have no rmal canilicular structures. At 90 days, the parietal bone of transgenic ma les was of normal width, suggesting that the original defect in matrix depo sition had been repaired or compensated for. In contrast, transgenic female s still had decreased bone mass in the parietal bone at 30 days. The decrea sed bone mass in TG females was accompanied by increased staining for osteo clast activity, suggesting that there was a seu-specific defect in mature a nimals. (C) 2000 Academic Press.