Structural studies of the O-specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92

Citation
J. Czaja et al., Structural studies of the O-specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92, EUR J BIOCH, 267(6), 2000, pp. 1672-1679
Citations number
33
Categorie Soggetti
Biochemistry & Biophysics
Journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
ISSN journal
00142956 → ACNP
Volume
267
Issue
6
Year of publication
2000
Pages
1672 - 1679
Database
ISI
SICI code
0014-2956(200003)267:6<1672:SSOTOP>2.0.ZU;2-3
Abstract
The structure of the O-specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by N MR spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded tha t the polysaccharide is composed of a hexasaccharide repeating unit with th e following structure: [GRAPHICS] in which D-beta-D-Hepp is D-glycero-beta-D-manno-heptopyranose and 6d-beta- d-Hep is 6-deoxy-beta-D-manno-heptopyranose. This structure represents a no vel hexasaccharide repeating unit of bacterial O-antigen that is characteri stic and unique to the Plesiomonas shigelloides strain. Using the high-reso lution magic angle spinning technique, H-1-NMR spectra were also obtained f or the O-polysaccharide components of isolated LPS and in their original fo rm directly on the surface of bacterial cells.