J. Czaja et al., Structural studies of the O-specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92, EUR J BIOCH, 267(6), 2000, pp. 1672-1679
The structure of the O-specific side chain of the lipopolysaccharide (LPS)
of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by N
MR spectroscopy, matrix-assisted laser desorption/ionization time of flight
mass spectrometry and sugar and methylation analysis. It was concluded tha
t the polysaccharide is composed of a hexasaccharide repeating unit with th
e following structure:
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in which D-beta-D-Hepp is D-glycero-beta-D-manno-heptopyranose and 6d-beta-
d-Hep is 6-deoxy-beta-D-manno-heptopyranose. This structure represents a no
vel hexasaccharide repeating unit of bacterial O-antigen that is characteri
stic and unique to the Plesiomonas shigelloides strain. Using the high-reso
lution magic angle spinning technique, H-1-NMR spectra were also obtained f
or the O-polysaccharide components of isolated LPS and in their original fo
rm directly on the surface of bacterial cells.