Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Authors
Wang, Y Liu, Y Sarker, KP Nakashima, M Serizawa, T Kishida, A Akashi, M Nakata, M Kitajima, I Maruyama, I
Citation
Y. Wang et al., Polymyxin B binds to anandamide and inhibits its cytotoxic effect, FEBS LETTER, 470(2), 2000, pp. 151-155
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
470
Issue
2
Year of publication
2000
Pages
151 - 155
Database
ISI
SICI code
0014-5793(20000324)470:2<151:PBBTAA>2.0.ZU;2-G
Abstract
Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to he a paracrine contrib utor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol, We confirmed the direct binding of PMB to A NA by using surface plasmon resonance. The adsorption of ANA by PMB may abo lish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin sho ck. (C) 2000 Federation of European Biochemical Societies.