Preparation and cDNA sequence analysis of two novel monoclonal antibodies against magaininII

Citation
Ts. Hu et al., Preparation and cDNA sequence analysis of two novel monoclonal antibodies against magaininII, HYBRIDOMA, 19(1), 2000, pp. 95-99
Citations number
17
Categorie Soggetti
Immunology
Journal title
HYBRIDOMA
ISSN journal
0272457X → ACNP
Volume
19
Issue
1
Year of publication
2000
Pages
95 - 99
Database
ISI
SICI code
0272-457X(200002)19:1<95:PACSAO>2.0.ZU;2-G
Abstract
By using intrasplenic immunization and the conventional B lymphocyte hybrid oma technique, we have established two novel hybridoma cell lines stably se creting specific monoclonal antibodies (MAbs) to magaininII, termed as 2D1 and 3F8, respectively, The two cell lines were then subjected to RNA extrac tion and the V-H and V-L segments were obtained by reverse transcription of RNA followed by polymerase chain reaction (RT-PCR) and characterized by nu cleotide sequence analysis. The V-H segments of 2D1 and 3F8 belong to the V H5 family and the V-L segments of 2D1 and 3F8 belong to V(K)10 and V(K)1 gr oups, respectively. The two MAbs utilize different V-L segments and have di sparities in their HCDR3 regions, which may contribute to the different epi tope recognition of the two antibodies.