Campylobacter-like organisms were isolated from the faeces of healthy indiv
iduals during a hygiene survey of abattoir workers. The strains, which exhi
bited characteristics of Campylobacter, being non-glucose-fermenting. oxida
se- and catalase-positive, Gram-negative, motile rods, were identified to t
he genus level by a PCR assay. Nucleotide sequence analysis of the 16S rRNA
gene, DNA homology experiments and determination of G+C content demonstrat
ed that they constituted a previously undescribed species, whose nearest ph
ylogenetic neighbours were Campylobacter hyointestinalis subsp, hyointestin
alis, Campylobacter fetus and Campylobacter mucosalis, The name Campylobact
er lanienae sp. nov. is proposed for this taxon and species-specific PCR pr
imers were evaluated which will find use in the study of its epidemiology,
prevalence and pathogenicity.