Identification of smoked Atlantic salmon (Salmo salar) and rainbow trout (Onchorhynchus mykiss) using PCR-restriction fragment length polymorphism ofthe p53 gene

Raw and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout ( Oncorhynchus mykiss) were identified using polymerase chain reaction-restri ction fragment length polymorphism (PCR-RFLP) of the p53 gene. DNA from S. salar and O. mykiss was amplified by using primers flanking exons 5 to 6 of the p53 nuclear gene. PCR products of different length were obtained for e ach species (532 and 518 base pairs, respectively). Sequences of PCR produc ts obtained from S, salar and O. mykiss were compared in the search for pol ymorphic restriction sites. The restriction fragments obtained with Eco RV, Hinf l, and Tao l endonucleases showed interspecific polymorphism, making it a useful method for identification of Atlantic salmon and rainbow trout.