Inositol monophosphates prepared in a single step from myo-inositol and inorganic phosphate

A mixture of myo-inositol and inorganic metaphosphate (prepared by freeze d rying an aqueous solution at pH 4) produced, upon warming, in moderate yiel d every inositol monophosphate ester, thereby providing in a single step a complete library of these compounds. The individual inositol monophosphates , both meso-compounds and racemic mixtures of the two pairs of enantiomers, were isolated by ion-exchange chromatography which was facilitated by the addition of berate to the mobile phase. The phosphates were characterised u sing phosphorus analysis' in conjunction with alkaline phosphatase, mass sp ectrometry and H-1 NMR spectroscopy.