Cleaning procedure for protein G affinity columns

An efficient method for cleaning protein G affinity columns is described in which impurities are removed effectively with a combination of 4 mol/1 ure a and 0.1 mol/1 sodium hydroxide. These cleaning conditions have only a min or deleterious effect on the binding capacity of the column and no effect o n its selectivity for IgG. This cleaning procedure should prolong the life of costly affinity columns used in the purification of immunoglobulin G (Ig G). (C) 2000 Published by Elsevier Science B.V. All rights reserved.