Cyclical changes in sperm volume during in vitro incubation under capacitating conditions: a novel boar semen characteristic

Citation
Am. Petrounkina et al., Cyclical changes in sperm volume during in vitro incubation under capacitating conditions: a novel boar semen characteristic, J REPR FERT, 118(2), 2000, pp. 283-293
Citations number
36
Categorie Soggetti
da verificare
Journal title
JOURNAL OF REPRODUCTION AND FERTILITY
ISSN journal
00224251 → ACNP
Volume
118
Issue
2
Year of publication
2000
Pages
283 - 293
Database
ISI
SICI code
0022-4251(200003)118:2<283:CCISVD>2.0.ZU;2-S
Abstract
The osmotic reactivity of boar spermatozoa during incubation in vitro was s tudied using a hypo-osmotic swelling test in conjunction with electronic me asurement of cell volume. Sperm populations showed fluctuations in both iso -osmotic cell volume and hypo-osmotic volume response that fitted mathemati cal models for periodicity. Significant differences of frequency and amplit ude were observed during sperm incubation under capacitating conditions as compared. with those under noncapacitating conditions. In addition, differe nt boars showed specific differences in their fluctuation characteristics u nder capacitating conditions. During incubation under capacitating conditio ns, a decrease in osmotic reactivity was observed that correlated with a de crease in motility, while the absolute value of the earliest maximum of the osmotic-induced response correlated with an increase in the proportion of discharged acrosomes. The time course of the cyclical behaviour of osmotic reactivity may be a useful parameter for assessing boar sperm response to c apacitating conditions.