Rhodopsin is the seven transmembrane helix receptor responsible for di
m light vision in vertebrate rod cells. The protein has structural hom
ology with the other G protein-coupled receptors, which suggests that
the tertiary structures and activation mechanisms are likely to be sim
ilar. However, rhodopsin is unique in several respects. The most strik
ing is the fact that the receptor ''ligand'', 11-cis retinal, is coval
ently bound to the protein and is converted from an ''antagonist'' to
an ''agonist'' upon absorption of light. NMR studies of rhodopsin and
its primary photoproduct, bathorhodopsin, have generated structural co
nstraints that enabled docking of the 11-cis and all-trans retinal chr
omophores into a low-resolution model of the protein proposed by Baldw
in. These studies also suggest a mechanism for how retinal isomerizati
on leads to rhodopsin activation. More recently, mutagenesis studies h
ave extended these results by showing how the selectivity of the retin
al-binding site can be modified to favor the all-trans over the 11-cis
isomer. The structural constraints produced from these studies, when
placed in the context of a high-resolution model of the protein, provi
de a coherent picture of the activation mechanism, which we show invol
ves a direct steric interaction between the retinal chromophore and tr
ansmembrane helix 3 in the region of Gly121. (C) 1997 Academic Press L
imited.