ATP-dependent chromatin-remodeling complexes are conserved among all eukary
otes and function by altering nucleosome structure to allow cellular regula
tory factors access to the DNA. Mammalian SWI-SNF complexes contain either
of two highly conserved ATPase subunits: BRG1 or BRM. To identify cellular
genes that require mammalian SWI-SNF complexes for the activation of gene e
xpression, we have generated cell lines that inducibly express mutant forms
of the BRG1 or BRM ATPases that are unable to bind and hydrolyze ATP. The
mutant subunits physically associate with at least two endogenous members o
f mammalian SWI-SNF complexes, suggesting that nonfunctional, dominant nega
tive complexes may be formed. We determined that expression of the mutant B
RG1 or BRM proteins impaired the ability of cells to activate the endogenou
s stress response gene hsp70 in response to arsenite, a metabolic inhibitor
, or cadmium, a heavy metal. Activation of hsp70 by heat stress, however, w
as unaffected. Activation of the heme oxygenase 1 promoter by arsenite or c
admium and activation of the cadmium-inducible metallothionein promoter als
o were unaffected by the expression of mutant SWI-SNF components. Analysis
of a subset of constitutively expressed genes revealed no or minimal effect
s on transcript levels. We propose that the requirement for mammalian SWI-S
NF complexes in gene activation events will be specific to individual genes
and signaling pathways.