State-dependent effects of some neuropeptides and neurotransmitters on neuronal activity of the medial septal area in brain slices of the ground squirrel, Citellus undulatus

Neuronal activity of the medial septal area was recorded extracellularly in brain slices taken from hibernating (winter) and waking (summer) ground sq uirrels. The effects of neuropeptides identified in the brain tissue of hib ernators (Thr-Ser-Lys-Tyr, Thr-Ser-Lys-Tyr-Arg and Asp-Tyr) on the backgrou nd activity and responses to electrical stimulation of the median forebrain bundle were analysed. For comparison, the effects of bath application of n oradrenaline and serotonin were also tested. Spontaneous activity in half o f all neurons (47-56%) was changed under the influence of neuropeptides in hibernating ground squirrels, while in waking ground squirrels the proporti on of responsive neurons was significantly lower (25-30%). The tendency for higher efficacy in hibernating ground squirrels was observed for serotonin ; only noradrenaline was equally effective in both groups of animals. Elect rically evoked responses of the medial septal nucleus-nucleus of the diagon al band neurons were also strongly modulated by neuropeptides; their change s could occur in the absence of shifts in the level and pattern of spontane ous activity. All three neuropeptides had differential action on the level of spontaneous activity, as well as on inhibitory and excitatory components of electrically evoked responses. Thus, the character and distribution of the effects were state dependent and differed greatly in hibernating and wa king ground squirrels. The experiments confirmed that medial septal nucleus -nucleus of the diagonal band neurons have higher excitability and responsi veness to some neuropeptides and neurotransmitters in hibernating ground sq uirrels. The data obtained suggest an increased latent excitability and responsivene ss of septal neurons during hibernation and their possible active participa tion in urgent arousal under the influence of sensory signals. (C) 2000 IBR O. Published by Elsevier Science Ltd.