Hyperglycemia alone does not inhibit secretin-induced pancreatic bicarbonate secretion

Administration of exogenous insulin (INS) inhibits secretin-stimulated panc reatic bicarbonate (HCO3) output via a dose-dependent, neurally mediated me chanism. To determine whether this effect was due to systemic hyperinsuline mia or to reduced endogenous insulin production, we examined the effect of hyperglycemia on secretin-stimulated pancreatic secretion. Chronic pancreat ic fistulae were created in six dogs. After 30 minutes of equilibration, a computer-assisted hyperglycemic clamp protocol was used to maintain glucose (GLU) levels 100 or 150 mg/dL above basal in clamp animals; control animal s received volume- and rate-matched infusions of 0.9% saline. One hour afte r beginning the clamp period, intravenous secretin dose-response (16-125 ng /kg/h) was begun, doubling the dose every half hour. Unstimulated (0-30 min utes) HCO3, GLU, and INS levels did not differ between groups. INS and GLU levels in clamp animals were significantly elevated during clamp (30-90 min utes) and stimulated (90-210 minutes) periods. For the same periods, HCO3 s ecretion was not significantly changed despite profound hyperinsulinemia. W e conclude that systemic hyperinsulinemia alone does not inhibit secretin-s timulated HCO3 output. Since exogenous INS exerts feedback regulation on th e pancreas, we propose that suppression of endogenous INS secretion mediate s the previously reported inhibitory response.