Interferon-gamma induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases

Bidirectional signalling between Trypanosoma brucei brucei and CD8(+) T cel ls involves reciprocal action of the parasite-derived trypanosome derived l ymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretio n is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving m aximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretio n was dependent on tyrosine protein kinase activity. Specific blockers of t his signalling system resulted in lower yields of TLTF in the culture super natants.