Profile and kinetics of L-type calcium current during the cardiac ventricular action potential compared in guinea-pigs, rats and rabbits

The substantial interspecies differences in mammalian cardiac action potent ials (APs) are attributed primarily to variations in K+ currents. In a comp arative study On isolated ventricular myocytes from guinea-pigs, rabbits an d rats, we investigated the influence of the species-based AP differences o n the time course of the L-type Ca2+ current (I-Ca.L). In addition, we test ed whether also species-dependent properties of the I-Ca.L contribute to it s time course during the AP. In patch-clamp measurements, I-Ca.L was charac terised using conventional square pulses and digitised APs as command volta ges. Special interest was directed to the analysis of the actual time cours es of I-Ca.L, and L-type channel activation and inactivation during APs. Al though species-specific differences in AP shape strongly influence the ampl itude and the time course of I-Ca.L, divergence in L-type channel inactivat ion was found as well. In each species I-Ca.L inactivation was related to C a2+ influx via L-type channels. However, while L-type channels showed simil ar Ca2+ dependency in the rabbit and the rat. a 2-times higher Ca2+ influx was necessary to achieve a given degree of inactivation in the guinea-pig. Thus, inactivation of I-Ca.L is delayed in the guinea-pig, thereby contribu ting to the prolonged AP plateau in this species. Comparing the actual time course of I-Ca.L, and L-type channel activation and inactivation revealed that, in each species. fading of I-Ca.L during final repolarisation was cau sed by deactivation (i.e. closure of the channel's d-gate) rather than inac tivation.