Production of triploid plants of mulberry (Morus alba L) by endosperm culture

A continuously growing callus was obtained from immature endosperm of Mor u s alba L Cv S-36 cultured on Murashige and Skoog medium containing 5 mu m 2 ,4-dichlorophenoxyacetic acid. Shoot buds were produced when the callus was subcultured on a medium containing a cytokinin or a cytokinin and 1-naphth aleneacetic acid (NAA), The maximum number of shoots was formed on the medi um containing thidiazuron (1 mu M), or benzylaminopurine (5 mu M) and NAA ( 1 mu M). Shoots were multiplied by forced axillary branching and rooted in vitro. Endosperm-derived plants were established in soil. Each of the ten p lants examined cytologically was triploid (3n=42).