Eicosanoid generation and responsiveness of human lymphatics in hyperlipoproteinemia

In this work, the oxidation injury in hyperlipoproteinemia (HLP) was determ ined by measuring the isoprostane 8-epi-prostaglandin (PG) F-2 alpha in hum an lymphatics, lymph fluid, plasma, serum and urine. Lymphatics from 6 pati ents with HLP generated less PGI(2) and contained more 8-epi-PGF(2 alpha) a s compared to 6 normolipemics without risk factors. Likewise, plasma (29.3 vs 19.7 pg/ml), lymph fluid (137.3 vs 65.3 pg/ml), serum (286.7 vs 204.1 pg /ml) and urinary (360.8 vs 241.0 pg/mg creatinine) values of 8-epi-PGF(2 al pha) in HLP (as compared to normolipemics) were significantly elevated. Lym phatics from HLP showed an enhanced contractile response, less C-14-arachid onic acid conversion to PGI(2) and less PGI(2)-formation upon various stimu li compared to normolipemics of comparable age. These findings indicate tha t HLP-induced oxidation injury, resulting in an altered (iso-)eicosanoid pr oduction and function, may also significantly affect (patho-) physiology of lympathics. (C) 2000 Harcourt Publishers Ltd.