Production of reactive oxygen species by phagocytic cells after exposure to glass wool and stone wool fibres - effect of fibre preincubation in aqueous solution
T. Zoller et Wj. Zeller, Production of reactive oxygen species by phagocytic cells after exposure to glass wool and stone wool fibres - effect of fibre preincubation in aqueous solution, TOX LETT, 114(1-3), 2000, pp. 1-9
The potential of four man-made vitreous fibres (MMVFs) (glass wool Code A,
stone wool Code G, HT-N and MMVF 21) and of two natural mineral fibres (cro
cidolite, erionite) to induce production of reactive oxygen species (ROS) b
y differentiated HL-60 cells (HL-60-M cells) was investigated by determinat
ion of luminol-enhanced chemiluminescence (CL). Quartz served as positive c
ontrol. The same system was used to uncover possible influences of fibre pr
eincubation in aqueous solutions on the ROS-generating potential. Following
preincubation in unbuffered saline over about 4 weeks, Code A and G fibres
showed decreased ROS-generating potential as compared to freshly suspended
fibres. On the other hand, MMVF 21 and HT-N fibres as well as crocidolite
and erionite showed no decreased CL after incubation in aqueous solutions.
The observed decrease of the ROS-generating potential of Code A and G fibre
s after preincubation may be an expression of fibre surface alterations (le
aching, initiation of dissolution) that influences the response of exposed
phagocytic cells. After incubation of both fibres in buffered solutions at
different pH values (5.0, 7.3) a reduced ROS-generating potential was still
discernible as compared to freshly suspended fibres. (C) 2000 Elsevier Sci
ence Ireland Ltd. All rights reserved.