Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

We have previously linked the sorting signals of the lysosome-associated me mbrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/ LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia vi rus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Si g/E7/LAMP-1 can further enhance immune responses, sequential vaccination wi th Sig/E7/ LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that primin g with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8+ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials. (C) 20 00 Elsevier Science Ltd. All rights reserved.