Ten blood samples randomly collected from cows on a farm nearby Antwerp, Be
lgium, were inoculated into KIVI culture medium (Kit for In Vitro Isolation
of trypanosomes) and RPMI 10% + feeder medium. Within 3 weeks of incubatio
n all KIVI cultures and four RPMI 10% + feeder revealed presence of Trypano
soma theileri. Same practical implications regarding the use of KIVI for is
olation of pathogenic African trypanosomes from cattle and other Bovidae ar
e discussed. (C) 2000 Elsevier Science B.V. All rights reserved.