[1,2 C-13(2)]glutamine and [ring-H-2(5)]phenylalanine were infused for 7 h
into five postabsorptive healthy subjects on two occasions. On one occasion
, the tracers were infused intravenously for 3.5 h and then by a nasogastri
c tube for 3.5 h. The order of infusion was reversed on the other occasion.
From the plasma tracer enrichment measurements at plateau during the intra
venous and nasogastric infusion periods, we determined that 27 +/- 2% of th
e enterally delivered phenylalanine and 64 +/- 2% of the glutamine were rem
oved on the first pass by the splanchnic bed. Glutamine flux was 303 +/- 8
mu mol.kg(-1).h(-1). Of the enterally delivered [C-13]glutamine tracer, 73
+/- 2% was recovered as exhaled CO2 compared with 58 +/- 1% of the intraven
ously infused tracer. The fraction of the enterally delivered tracer that w
as oxidized specifically on the first pass by the splanchnic bed was 53 +/-
2%, comprising 83% of the total tracer extracted. From the appearance of C
-13 in plasma glucose, we estimated that 7 and 10% of the intravenously and
nasogastrically infused glutamine tracers, respectively, were converted to
glucose. The results for glutamine flux and first-pass extraction were sim
ilar to our previously reported values when a [2-N-15]glutamine tracer [Mat
thews DE, Morano MA, and Campbell RG, Am J Physiol Endocrinol Metab 264: E8
48-E854, 1993] was used. The results of [C-13]glutamine tracer disposal dem
onstrate that the major fate of enteral glutamine extraction is for oxidati
on and that only a minor portion is used for gluconeogenesis.