Effect of carboxymethylcellulose and a hyaluronate-carboxymethylcellulose membrane on healing of intestinal anastomoses in horses

Citation
Poe. Mueller et al., Effect of carboxymethylcellulose and a hyaluronate-carboxymethylcellulose membrane on healing of intestinal anastomoses in horses, AM J VET RE, 61(4), 2000, pp. 369-374
Citations number
32
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
ISSN journal
00029645 → ACNP
Volume
61
Issue
4
Year of publication
2000
Pages
369 - 374
Database
ISI
SICI code
0002-9645(200004)61:4<369:EOCAAH>2.0.ZU;2-C
Abstract
Objective-To evaluate the effect of sodium carboxymethylcellulose (SCMC) or a hyaluronate-carboxymethylcellulose membrane (HA membrane) on healing of the small intestine in horses. Animals-18 healthy adult horses. Procedure-Midline celiotomy and 2 jejunal resection-and-anastomosis surgeri es were performed. In treated horses, SCMC (n = 6) or a HA membrane (6) was applied to the jejunum to cover the anastomosis. There were 6 untreated co ntrol horses. Horses were euthanatized 10 days after surgery. For each hors e, 1 anastomosis was used for histologic examination, and the second was us ed to determine intestinal bursting strength. Intestinal bursting tension, serosal granulation tissue, serosal fibrin deposition, and width of the fib rous seal at the anastomosis were compared among groups. Results-3 control horses had adhesions associated with the anastomosis; but none of the treated horses had adhesions associated with the anastomosis. Mean thickness of fibrin deposited on the serosal surfaces for the SCMC and HA-membrane groups was significantly less than that for control horses. Me an thickness of serosal granulation tissue, width of fibrous seal between i nverted musculature, inflammatory cell infiltrate scores, and bursting tens ion did not differ significantly among groups. Conclusions and Clinical Relevance-Use of SCMC or application of a HA membr ane to small intestinal anastomoses in horses resulted in fewer adhesions a nd decreased fibrin deposition, and it did not adversely affect anastomotic healing. In horses at increased risk for intra-abdominal adhesions, SCMC o r application of HA membranes may decrease the frequency of adhesions witho ut adversely affecting healing of small intestinal anastomoses.