Identification of genetic product - GM-CSF by HPLC-ESIMS

The genetic product, granulocyte-macrophage colony stimulating-factor (GM-C SF) biosynthesized in this university, is identified through techniques of HPLC and ESIMS in the present study. Results indicate that the experimental molecular weight for the product protein is in agreement with the expected one. Partial sequences are inferred from the tryptic peptide map of produc t protein. Combining the partial sequence and molecular weights of tryptic peptides, the product protein of GM-CSF has been verified through the Swiss -Prot protein database with satisfactory results.