Fast amperometric determination of enzymatic activity of glutaminase

The activity of the enzyme glutaminase has been measured using a glutamate electrochemical biosensor based on H2O2 detection. Calibration curves for g lutamate detection and for glutaminase activity using standard glutaminase from Escherichia coli demonstrated the high sensitivity and the rapid analy sis time of this novel amperometric procedure, which was 100 times more sen sitive than that reported in literature. Porcine liver and kidney tissue and human kidney tissue samples have been t ested for glutaminase activity, demonstrating the possibility to perform me asurements directly on whole tissues, with no need of sample extraction and purification.