S. Klein et F. Ellendorff, Localisation of Xho1 repetitive sequences on autosomes in addition to the W chromosome in chickens and its relevance for sex diagnosis, ANIM GENET, 31(2), 2000, pp. 104-109
Repetitive sequences of the Xho1 family comprise about 70% of the W chromos
ome in chickens, are considered to be restricted to this sex chromosome and
are used in sex diagnosis. This study investigated the reliability of sex
diagnosis on small cell numbers. Fluorescent in situ hybridisations (FISH)
with the Xho1 repeat sequence were performed on metaphases and signals were
detected on the W chromosome and on some autosomes. Differences in signal
intensity suggested that the number of repeats ranged from several thousand
on the W chromosome to only a few on the autosomes. These results confirm
the report from Clinton (1994) of minor male products in sex specific PCR o
f the Xho1 repeat. We adopted his PCR protocol to analyse small numbers of
cells. FISH on blastomeres and on blood cells from E3 to E11 embryos were c
ompared to PCR results. The FISH procedure as well as the PCR protocol are
suitable for sexing. For single cells FISH was slightly more consistent.