A tetrodotoxin-producing Vibrio strain, LM-1, from the puffer fish Fugu vermicularis radiatus

Authors
Lee, MJ Jeong, DY Kim, WS Kim, HD Kim, CH Park, WW Park, YH Kim, KS Kim, HM Kim, DS
Citation
Mj. Lee et al., A tetrodotoxin-producing Vibrio strain, LM-1, from the puffer fish Fugu vermicularis radiatus, APPL ENVIR, 66(4), 2000, pp. 1698-1701
Citations number
33
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
4
Year of publication
2000
Pages
1698 - 1701
Database
ISI
SICI code
0099-2240(200004)66:4<1698:ATVSLF>2.0.ZU;2-H
Abstract
Identification of tetrodotoxin (TTX) and its derivatives produced from a Vi brio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, highperformanc e liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fis h.