Method for rapid purification of class IIa bacteriocins and comparison of their activities

Authors
Guyonnet, D Fremaux, C Cenatiempo, Y Berjeaud, JM
Citation
D. Guyonnet et al., Method for rapid purification of class IIa bacteriocins and comparison of their activities, APPL ENVIR, 66(4), 2000, pp. 1744-1748
Citations number
32
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
4
Year of publication
2000
Pages
1744 - 1748
Database
ISI
SICI code
0099-2240(200004)66:4<1744:MFRPOC>2.0.ZU;2-A
Abstract
A three-step method nas developed for the purification of mesentericin Y105 (60% yield) from the culture supernatant of Leuconostoc mesenteroides Y105 . The same procedure was successfully applied to the purification of fire o ther anti-Listeria bacteriocins identified by mass spectrometry. Specific a ctivities of the purified bacteriocins were compared.