C. Picherit et al., Dihydrotestosterone prevents glucocorticoid-negative effects on fetal rat metatarsal bone in vitro, BIOL NEONAT, 77(3), 2000, pp. 181-190
The effects of dihydrotestosterone (DHT) on glucocorticoid-pretreated fetal
rat long bone were studied in an in vitro culture system. First, dose-resp
onse curves of corticosterone, hydrocortisone, and dexamethasone were studi
ed at several concentrations. Then, hydrocortisone (H) at 10(-5) M was sele
cted for the second part of the study, as it slackened rudiment mineralizat
ion (104 +/- 16% of the initial dark zone vs. 141 +/- 9% in control bones),
as well as its lengthening (140 +/- 4% of the harvesting day length vs. 16
0 +/- 1% in control bones), by both inhibition of cell proliferation and st
imulation of resorption, On the contrary, in H-pretreated metatarsal bones,
DHT (10(-7) M) partly limited slackening of mineratization (124 +/- 5%) an
d lengthening (153 +/- 2%). Moreover, a control-like cell proliferation was
re-established and resorption holes were filled in. Thus, in this study, D
HT partly limited hydrocortisone-induced impairment of fetal rat metatarsal
bone development. Copyright (C) 2000 S. Karger AG, Basel.