Cell membrane changes of structure and function in protein kinase inhibitor-induced polyploid cells

Citation
Zp. Zong et al., Cell membrane changes of structure and function in protein kinase inhibitor-induced polyploid cells, CELL PROLIF, 33(1), 2000, pp. 29-38
Citations number
25
Categorie Soggetti
Cell & Developmental Biology
Journal title
CELL PROLIFERATION
ISSN journal
09607722 → ACNP
Volume
33
Issue
1
Year of publication
2000
Pages
29 - 38
Database
ISI
SICI code
0960-7722(200002)33:1<29:CMCOSA>2.0.ZU;2-1
Abstract
Exogenous cyclic AMP has been thought to be a chemical without marked pharm acological effect until now, as it is not capable of penetrating the cell m embrane in most eucaryotic cells. The present study obtained results consis tent with those of most previous studies, showing that exogenous cyclic AMP itself did not interfere with the cell cycle even at the high dose of 100 mu M. However, it was found that K252a, a potent inhibitor of protein kinas es including protein kinase C, induced DNA re-replication, i.e. DNA synthes is at a elevated DNA ploidy in cells that had not undergone cytokinesis (le ading to polyploidization), and that exogenous cyclic AMP markedly potentia ted the K252a-induced polyploidization at a very low dose similar to the ef fective dose of membrane-permeable cyclic AMP analogue dibutyryl cyclic AMP . These findings suggested that the cell membrane changed during the format ion of polyploid cells. This supposition was confirmed by scanning electron microscopy to observe structural changes and by determination of cellular attachment to investigate functional changes.