Sm. Cormier et al., Estimation of exposure criteria values for biliary polycyclic aromatic hydrocarbon metabolite concentrations in white suckers (Catostomus commersoni), ENV TOX CH, 19(4), 2000, pp. 1120-1126
A methodology was developed for deriving quantitative exposure criteria use
ful for comparing a site or a watershed to a reference condition and for de
fining the occurrence of extreme exposures. The prototype method used indic
ators of exposures to oil contamination and combustion by-products, naphtha
lene (NAPH)-type and benzo[a]pyrene (BaP)-type biliary metabolites from whi
te suckers (Catostomus commersoni). The fish were collected from first- to
third-order streams in the Regional Environmental Monitoring Assessment Pro
gram (REMAP) study of the Eastern Corn Belt Plains ecoregion at randomly se
lected sires and from third- or higher-order streams in conjunction with th
e Ohio Environmental Protection Agency (OEPA) Biomonitoring Program. which
sampled sites of concern and reference sites selected by ecologists. The po
lycyclic aromatic hydrocarbon metabolites were measured using fixed-wavelen
gth fluorescence with excitation/emission pairs at 290/335 nm for NAPH-type
and 380/430 nm for BaP-type metabolites. Exposure criteria values were sel
ected for each type of metabolite for both the REMAP study and the OEPA ref
erence sites following the recommendations of the International Federation
of Clinical Chemistry. Exposure criteria derived for each metabolite were n
ot significantly different for REMAP and OEPA reference sites. More than on
e half of the OEPA nonreference sites were found to be contaminated, exceed
ing criteria values for both types of metabolites. This method for finding
meaningful exposure criteria can be used to develop criteria of exposure to
other contaminants for other wildlife and other ecosystems.