Am. Castillo et al., Comparison of anther and isolated microspore cultures in barley. Effects of culture density and regeneration medium, EUPHYTICA, 113(1), 2000, pp. 1-8
A comparison of anther and microspore culture efficiency for green doubled
haploid plant production was undertaken using 17 F-1 crosses with potential
agronomic performance. Green doubled haploid plants were produced from all
F-1 crosses by anther and microspore cultures, although there was a great
variation among crosses. On average, anther culture resulted in a productio
n of green plant twice that of isolated microspore culture (30 and 14 green
plants/100 anthers, respectively). The effect of microspore culture densit
y on green plant regeneration was studied with the cultivars Igri, Reinette
and Hop which have a high, medium and low androgenic response. The highest
number of dividing microspores was obtained at a density of 2.4 x 10(5) vi
able microspores/ml for the three cultivars. However, the optimal density f
or the percentage of embryos/dividing microspores and green plants/10(3) mi
crospores depended on the cultivar. The highest number of green plants/10(3
) microspores was produced at 1.2 x 10(5) viable microspores/ml for cv. Igr
i and 2.4 x 10(5) for cultivars Reinette and Hop. Percentage of green plant
s/total plants was raised when the culture density was increased up to 6.0
x 10(5) viable microspores/ml, especially for cv. Reinette. Six regeneratio
n media differing in maltose concentration, organic nitrogen and type of au
xin were assayed with embryos from cultivar Reinette. Media without organic
nitrogen containing 31 g l(-1) maltose and the auxins IAA or NAA produced
more vigorous green plants.