Biomass conversion: Synergistic use of immobilized alpha-amylase and amyloglucosidase for rapid and maximum conversion of starch into glucose

Enzyme technology is developed for the conversion of starch into glucose sy rup, containing 99% (w/w) glucose using synergistic action of alpha-amylase and amyloglucosidase. For repeated and efficient use of these enzymes, the y were covalently immobilized on agaropectin, which proved to be an ideal s upport for immobilization of these enzymes. Parameters for immobilized enzy mes were determined and compared with free enzymes. optimum enzyme starch r atio was determined for rapid and maximum conversion of starch into glucose . Attempts were made to improve quality of glucose by minimizing the revers ion product formation.