S. Chanteau et al., 45/47 kilodalton (APA) antigen capture and antibody detection assays for the diagnosis of tuberculosis, INT J TUBE, 4(4), 2000, pp. 377-383
Citations number
18
Categorie Soggetti
Cardiovascular & Respiratory Systems
Journal title
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
SETTING: APA complex (45/47 kDa) is an antigen specifically excreted by Myc
obacterium tuberculosis and could therefore be a good candidate for diagnos
is.
OBJECTIVES: TO develop three APA immunocapture ELISA assays using monoclona
l antibodies (Mabs) and one IgG anti-APA ELISA test, and to determine their
usefulness for the diagnosis of tuberculosis in Madagascar.
DESIGN: For the Ag assays, 23 negative sputum and serum samples and 64 pair
s of sputum and serum from active smear-positive patients (PTM+) were teste
d. For antibody assay, 116 negative controls, 143 PTM+ and 54 extra-pulmona
ry tuberculosis patients were tested.
RESULTS: The sensitivities of the APA antigen detection assays were low (le
ss than 40%) for a specificity of 95.6%, using either monoclonal antibodies
or clinical specimens. The anti-APA serology was more sensitive (76.9% for
PTM+ patients) but less specific (73.2%). Due to their poor predictive val
ues, these tests cannot be recommended for the routine diagnosis of tubercu
losis in Madagascar.