Pooling of urine specimens for detection of asymptomatic Chlamydia trachomatis infections by PCR in a low-prevalence population: Cost-saving strategyfor epidemiological studies and screening programs

Pooling, in groups of five, of urine specimens from asymptomatically infect ed men in a population with 4% prevalence, as determined by case finding, i s 100% sensitive and specific and results in a 60.5% reduction in the numbe r of tests needed. Pooling of urine specimens in groups of 10 for the estim ation of population-based prevalence is 96.1% sensitive and 100% specific a nd saves 90% of the test costs.