Cryopreservation of Chrysanthemum cinerariaefolium shoot tips

A simple method for efficient cryopreservation of Chrysanthemum cinerariaef olium shoot tips is described. it comprises the following steps: treatment of the explanted shoot tips with 0.55 mol/L sucrose plus 4 mu mol/L ABA for 3 days, direct immersion in liquid nitrogen, rapid rewarming at 40 degrees C and recovery of the shoot tips on solid nutrient medium containing 11 mu moI/L ANA and 4.5 mu mol/L BAP. About 75% of the shoot tips withstood cryo preservation using this procedure. The morphogenic potential of frozen shoo t tips, and total chlorophyll and pyrethrin contents of regenerated plants were preserved after the different treatments.