The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin

Authors
Garcia-Ortega, L Lacadena, J Lacadena, V Masip, M de Antonio, C Martinez-Ruiz, A del Pozo, AM
Citation
L. Garcia-ortega et al., The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin, LETT APPL M, 30(4), 2000, pp. 298-302
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
30
Issue
4
Year of publication
2000
Pages
298 - 302
Database
ISI
SICI code
0266-8254(200004)30:4<298:TSOTRA>2.0.ZU;2-F
Abstract
The yield of purified recombinant alpha-sarcin increases approximately thre e- to fourfold when this toxin is co-expressed in Escherichia coli with thi oredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangeme nt of incorrect disulphide bonds to produce the soluble native conformation . The protein thus produced retains the structural, spectroscopic and enzym atic features of the natural fungal alpha-sarcin.