A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

Authors
McKerrow, J Vagg, S McKinney, T Seviour, EM Maszenan, AM Brooks, P Seviour, RJ
Citation
J. Mckerrow et al., A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria, LETT APPL M, 30(3), 2000, pp. 178-182
Citations number
25
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
30
Issue
3
Year of publication
2000
Pages
178 - 182
Database
ISI
SICI code
0266-8254(200003)30:3<178:ASHMFA>2.0.ZU;2-B
Abstract
A Simple and sensitive method for separating and detecting the LL, DD and m eso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in th e peptidoglycan of Gram-positive bacteria is described. This method is base d on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is describe d, where 10 mg or less of dry cells is required.