Oxidative stress-related proteins A170 and heme oxygenase-1 are differently induced in the rat cerebellum under kainate-mediated excitotoxicity

A170 and heme oxygenase-1 (HO-1) are characterized as oxidative stress-indu cible proteins whose induction depends upon common transcription factor via antioxidant responsive element. We investigated the expression of A170 and HO-1 in the cerebellum after kainate administration. In situ hybridization showed constitutive expression of A170 and HO-1 mRNA in Purkinje cell laye r; mild induction of A170 or HO-1 was detected, respectively, 8 or 24 h aft er kainate administration, Immunohistochemical studies also demonstrated th at constitutive expression and the induction of A170 protein in Purkinje ce lls; the induction of HO-1 protein was detected in Bergmann glia but not in Purkinje cells. Thus, the transcription factors involved in the induction of A170 might be different from those in the induction of HO-1 under kainat e-mediated excitotoxicity. The existence of cell type-specific stress respo nse was suggested. (C) 2000 Elsevier Science Ireland Ltd. All rights reserv ed.