Development of an isothermal titration microcalorimetric system with digital control and dynamic power peltier compensation. II. Characterization andoperation mode. Myoglobin adsorption onto polymeric latex particles

In a previous article a comprehensive description of an isothermal titratio n microcalorimeter with Peltier compensation was reported. This work deals with the characterization procedure and the operation mode. The transfer fu nction parameters (time constants, calibration constants, and thermal prope rties of the system components) have been determined using a rigorous physi cal model for the microcalorimeter. To check the good performance of the in strument, titration experiments of cytidine and adenosine protonation have been carried out. Finally, as an example of the instrument applicability, d ifferential heat measurements of myoglobin adsorption onto polymeric (polys tyrene) latex particles are presented. (C) 2000 American Institute of Physi cs. [S0034-6748(00)05104-2].