Ha. Sousa et al., Asymmetric hydrolysis of a meso-diester using pig liver esterase immobilised in hollow fibre ultrafiltration membrane, TETRAHEDR-A, 11(4), 2000, pp. 929-934
Pig liver esterase (PLE) was physically immobilised in a polysulphone ultra
filtration hollow fibre membrane reactor and used for the repetitive batch
two-phase hydrolysis and separation, on a multigram scale, of the meso-dies
ter dimethyl cis-cycloxex-4-ene-1,2-dicarboxylate 1 to enantiomerically pur
e (1S,2R)-cyclohex-4-ene1,2-dicarboxylic acid monomethyl ester 2, After 25
days, the enzyme still retained its initial activity, which corresponds to
62% of its activity in the free form, and the enantiomeric purity of monoes
ter 2 was still higher than 97%. Simple experimental conditions were establ
ished for the large laboratory scale preparation of substrate 1 and isolati
on of product 2 from the aqueous phase. (C) 2000 Elsevier Science Ltd. All
rights reserved.