Jo. Svensson et al., Determination of ribavirin in serum using highly selective solid-phase extraction and high-performance liquid chromatography, THER DRUG M, 22(2), 2000, pp. 215-218
A rapid assay for determination of ribavirin in serum using solid-phase ext
raction (SPE), high-performance liquid chromatography (HPLC), and UV-detect
ion was developed. The SPE uses phenylboronic acid columns with an approxim
ately 100% recovery for ribavirin. The concentration-peak area relation was
linear (r > 0.995), from 1 to 64 mu M in 100 mu L serum. The limit of dete
ction was 0.1 mu M. The intraassay CV was 3.2% at treatment levels (9.7 mu
M) and 11.5% at 0.4 mu M The method is used to monitor patients undergoing
ribavirin treatment for hepatitis C (HCV). Samples from HCV-infected patien
ts with and without renal dysfunction have been analyzed without interferen
ce of endogenous compounds. It is concluded that the method is useful for r
outine therapeutic drug monitoring.