The National AIDS Control Organization (NACO), the apex body for controllin
g AIDS in India, projected that HN seroprevalence would increase from 7/100
0 in 1995 to 21.2/1000 in 1997. A high incidence (8.2%) of HIV was observed
in blood donors. This study was carried out to find out the true HIV posit
ivity in Indian blood donors. Blood donors from our centre were followed fo
r more than 5 years to determine the true HIV seroprevalence and our result
was compared with similar studies from India. Voluntary and relative blood
donors who visited the SGPGIMS, Lucknow, since 1993 to June 1998 were incl
uded. They were screened for HIV 1/2 by ELISA kits (WHO approved). First-ti
me HIV-positive samples were preserved frozen for further study (stage-I).
They were repeated in duplicate and retested with other kits. If found posi
tive, the sample was labelled as ELISA positive (stage-II). ELISA-positive
samples were confirmed by Western Blot (WB) at stage-III. A total of 65 288
donors were included and 834 (12.8/1000) were reactive at stage-I. But 1.1
/1000 donors were found to be ELISA positive at stage-II, and 0.28/1000 don
ors were positive by WB at stage-III. The 'seropositivity' rate from the NA
CO was significantly (P < 0.001) higher than our study. There were five sim
ilar Indian studies and seropositivity rate varied from 0.72/1000 (using EL
ISA and WE) to 5.5/1000 (using ELISA alone). The 'seropositivity' rate from
the NACO was significantly (P < 0.001) higher than all these studies. HIV
seroprevalence in the present study is lower (P < 0.001) than other Indian
figures. The present and other studies confirmed that the projected HIV ser
oprevalence (82/1000) in Indian blood donors was high. The NACO result was
based on one-time ELISA screening reports from zonal blood testing centres
which also receive samples from paid donors donating in commercial blood ba
nks. The HIV prevalence of blood donors (and national prevalence) is to be
reassessed.