Effect of anti-digoxin antiserum on endoxin and membrane ATPase activity in hypoxia-reoxygenation induced myocardial injury

AIM: To evaluate the protective effect of anti-digoxin antiserum on hypoxia -reoxygenation induced injured myocardium and its mechanism. METHODS: Anti- digoxin antiserum of different concentrations was used, its effect on endox in and ATPase activity in cell membrane in hypoxia-reoxygenation myocardium model was observed. RESULTS: The level of endoxin was remarkably higher, A TPase activities in cell membrane were remarkably lower in hypoxic group an d hypoxia-reoxygenation injury group than those of normal group; anti-digox in antiserum could resume ATPase activity in a concentration-dependent mann er. CONCLUSION: Rise of endoxin was the molecular biological basis of myoca rdial damage during myocardial hypoxia-reoxygenation. Anti-digoxin antiseru m had lessened myocardial injury and had a protective effect on hypoxia-reo xygenation myocardium by antagonizing effect of endoxin.