TNF alpha, IL-1 beta and IL-6 production by peripheral blood monocytes in patients with renal cell carcinoma

Renal cell carcinoma (RCC) has been shown to be immunologically more labile than other types of cancer. In this study, we examined tumor necrosis fact or alpha (TNF alpha) interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6 ) production of peripheral blood monocytes in 38 RCC patients. Monocytes we re isolated from peripheral blood mononuclear cells by adherence to a plast ic dish and cultured with lipopolysaccharide for 24 hours. The culture supe rnatant was obtained, and the production of TNF alpha, IL-1 beta and IL-6 w as measured by ELISA. As a result, TNF alpha and IL-1 beta production was s ignificantly higher in the high stage patients compared to the control subj ects and low stage patients. When the patients were divided according to se rum C-reactive protein (CRP), TNF alpha, IL-1 beta and IL-6 production was significantly higher in the CRP-positive patients compared to the control s ubjects and the CRP- negative patients. Overexpression of these cytokines m ay therefore induce a hypermetabolic status that may be a cause of malnutri tion and cancer cachexia. role in the regulation of the cellular and humora l immune response. Some aspects of this regulatory function are mediated by their soluble products such as tumor necrosis factor a (TNF alpha), interl eukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) (4). In this study, in or der to study the monocyte function in patients with RCC, we investigated th e production of TNF alpha, IL-1 beta and IL-6 by lipopolysaccharide (LPS)ac tivated blood monocytes.