Lr. Wedderburn et al., Selective recruitment of polarized T cells expressing CCR5 and CXCR3 to the inflamed joints of children with juvenile idiopathic arthritis, ARTH RHEUM, 43(4), 2000, pp. 765-774
Objective. To study the expression of chemokine receptors CCR5 and CXCR3 an
d the Th1/Th2 cytokine balance in children with oligoarticular or polyartic
ular juvenile idiopathic arthritis (JIA).
Methods. Using 3-color immunofluorescence, we studied the expression of CCR
5 and CXCR3 on, and T cell cytokine production by, paired samples of synovi
al fluid (SF) and peripheral blood (PB) T cells from 20 patients with oligo
articular- or polyarticular-onset JIA, Chemokine and cytokine phenotypes we
re also compared within the CD45RO+,CD3+ subsets. CCR5 genotypes were confi
rmed by polymerase chain reaction typing and sequencing.
Results. In the majority of samples, the number of T cells that were CCR5and CXCR3+ was higher in SF than in PB, and this difference was significant
. One child was homozygous for the null Delta 32 CCR5 allele; 4 others had
lower expression of CXCR3 in SF than in blood, All samples showed strongly
Th1-type cytokine production by synovial T cells compared with that by PB T
cells. Both features were also markedly polarized within the synovial CD45
RO+ subset compared with PB CD45RO+ T cells.
Conclusion. The high expression of CCR5 and CXCR3 and high interferon-gamma
:interleukin-4 ratios suggest a type 1 phenotype of SF T cells in JIA. The
difference between CD45RO+ T cells from SF and from PB suggests that specif
ic activation events have occurred in synovial T cells. We suggest that the
highly activated, Th1-type phenotype of T cells within the chronically inf
lamed joints of children with JIA may reflect specific recruitment events t
hat contribute to the polarization of these cells.