Inhibition of process outgrowth by tau antisense oligonucleotide in rat medullary thyroid carcinoma cells

Citation
I. Nishiyama et T. Oota, Inhibition of process outgrowth by tau antisense oligonucleotide in rat medullary thyroid carcinoma cells, BIOMED RES, 20(6), 1999, pp. 309-314
Citations number
26
Categorie Soggetti
Medical Research General Topics
Journal title
BIOMEDICAL RESEARCH-TOKYO
ISSN journal
03886107 → ACNP
Volume
20
Issue
6
Year of publication
1999
Pages
309 - 314
Database
ISI
SICI code
0388-6107(199912)20:6<309:IOPOBT>2.0.ZU;2-P
Abstract
Calcitonin-producing cells (C-cells) are neural crest derived endocrine cel ls with some intrinsic neuronal properties. Two types of protein kinase inh ibitors, H-7 and staurosporin, promoted marked outgrowth of neurite-like pr ocesses in the cells of rat neoplastic C-cell line, rMTC 6-23. The cells sh owed immunoreactivities to both alpha-tubulin and tau proteins in the whole cytoplasm including processes, suggesting that the microtubule cytoskeleto n in the processes is stabilized by tau proteins. To test the possible role of tau proteins in the process outgrowth, the cells were treated with sens e- and antisense-oriented deoxyoligonucleotides encoding regions of the tau sequence that overlap the translation initiation codon. Antisense, but not sense, oligonucleotide treatment reduced tau-immunoreactivity to backgroun d levels, and markedly inhibited H-7- or staurosporin-induced process outgr owth in the cells. These findings have clearly indicated that tau proteins participate in the outgrowth of neuritic processes in rMTC 6-23 cells.