Biosynthesis of L-lysine by Corynebacterium glutamicum grown on fish silage

The microbial utilization of fish silage as a fermentation substrate for th e extracellular production of L-lysine by Corynebacterium glutamicum ATCC 2 1543 was studied in Erlenmeyer baffled flasks. When culture media were supp lemented with fish silage at 40 g/l the concentrations of biomass and L-lys ine attained 10 and 30 g/l, respectively after 72 h of fermentation. Kineti c fermentation data. the specific rate of L-lysine production and the produ ct yield reached maximum values of 0.096 g/g h and 0.30 g/g, respectively, when yeast extract was replaced by fish silage in culture media. Statistica l analysis showed that there was not a significant difference (P > 0.05) be tween yeast extract (20 g/l) and fish silage (40 g/l) kinetic fermentation data. (C) 2000 Elsevier Science Ltd. All rights reserved.