DNA extraction method for screening yeast clones by PCR

A simple procedure for isolating yeast DNA suitable for use ns a template f or PCR amplification is described. SDS treatment alone is sufficient for ex traction of chromosomal DNA from yeast cells. Cells of a yeast colony are s uspended in a small volume (about 20 mu L) of a 0.25% SDS solution, mixed v igorously and centrifuged. The supernatant cart be directly used as a templ ate after dilution to give nn SDS concentration of less than 0.01% in the f inal PCR mixture.